ITS2 Isolation in Endemic Loka Pere Banana: A New Potential DNA Barcode Marker

Abstract The present research aimed to investigate Loka Pere banana (Musa sp.), an endemic species with distinct characteristics, but currently underrepresented in scientific literature. Our primary goal was isolate the Internal Transcribed Spacer (ITS2) region from leaf and corm samples of this unique variant. As a methodological approach, we utilized modified CTAB protocol extract DNA amplified ITS2 via polymerase chain reaction (PCR) using ITS-S2F ITS-S3R primers, achieving amplicon size approximately 500 bp long. results showed successful isolation sequences both Banana. This finding signifies potential as novel reliable barcode marker. Future can leverage data for more accurate molecular identification phylogenetic analyses, enhancing our understanding genetic diversity among Musa genus.